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Image Search Results
Journal: Nature Communications
Article Title: Defect engineering of layered double hydroxide nanosheets as inorganic photosensitizers for NIR-III photodynamic cancer therapy
doi: 10.1038/s41467-022-31106-9
Figure Lengend Snippet: a Biodistribution of DR-CoMo-LDH-PEG in mice by monitoring the Co concentration at various time points post-injection. Data are presented as mean values ± s.d. ( n = 3). Statistical analysis was performed via one-way ANOVA. *p < 0.05, **p < 0.01, ***p < 0.001. b Tumor growth curves of mice after various treatments. Data are presented as mean values ± s.d. ( n = 6). Statistical analysis was performed via one-way ANOVA. ***p < 0.001. c Representative photographs of mice with treatments at various time points and d corresponding average weight of tumors taken on Day 16. Data are presented as mean values ± s.d. ( n = 6). Statistical analysis was performed via one-way ANOVA. ***p < 0.001. e Body weight change of 4T1 tumor-bearing mice after different treatments. Data are presented as mean values ± s.d. ( n = 6). f HIF-1α, g DHE, and h H&E, Ki-67, TUNEL and CD31 staining assay of tumor slices from various groups of mice after 16 days of treatment: 1) PBS, 2) 1567 nm laser (0.5 W cm −2 for 6 min), 3) DR-CoMo-LDH-PEG, 4) DR-CoMo-LDH-PEG + 1567 nm laser. Each experiment was repeated three times with similar results. Source data are provided as a Source Data file.
Article Snippet: The tumor slices were stained with primary antibodies (
Techniques: Concentration Assay, Injection, TUNEL Assay, Staining
Journal: International Journal of Biological Sciences
Article Title: Over-activation of TRPM2 ion channel accelerates blood-spinal cord barrier destruction in diabetes combined with spinal cord injury rat
doi: 10.7150/ijbs.80672
Figure Lengend Snippet: Diabetes impedes neovascularization level in spinal cord of SCI rat. (A and B) Representative western blotting results and quantitative analysis of HIF-1α, ANG1 and VEGF in spinal cord at 3 days after SCI. (C and D) Representative western blotting results and quantitative analysis of HIF-1α, ANG1 and VEGF in HUVECs. (E) Co-staining of HIF-1α (green) and CD31 (red) in spinal cord at 3 days after SCI, Scale bar = 10 μm. (F) Co-staining of ANG1 (green) and CD31 (red) in spinal cord at 3 days after SCI, Scale bar = 10 μm. (G) The tube formation ability of HUVECs, Scale bar = 200μm. (H) Quantification of tube length from tube formation assay. n≥3, *P<0.05, **P<0.01, ***P<0.001.
Article Snippet: Then, the membranes were blocked with 5% skimmed milk in TBST for 2 h at room temperature, and incubated with the following primary antibodies at 4°C overnight: TRPM2 (1:1000, NB500-242), p-CaMKII (1:1000, sc-32289), CaMKII (1:1000, sc-5306), eNOS (1:1000, sc-376751), NOX2 (1:1000, ab129068),
Techniques: Western Blot, Staining, Tube Formation Assay
Journal: International Journal of Biological Sciences
Article Title: Over-activation of TRPM2 ion channel accelerates blood-spinal cord barrier destruction in diabetes combined with spinal cord injury rat
doi: 10.7150/ijbs.80672
Figure Lengend Snippet: TRPM2 inhibition improves angiogenesis level under diabetes combined with SCI condition. (A and B) Representative western blotting results and quantitative analysis of HIF-1α, ANG1 and VEGF in spinal cord of rat at 3 days after SCI. (C and D) Representative western blotting results and quantitative analysis of HIF-1α, ANG1 and VEGF in HUVECs. (E) Co-staining of HIF-1α (green) and CD31 (red) in spinal cord at 3 days after SCI, Scale bar = 10 μm. (F) Co-staining of ANG1 (green) and CD31 (red) in spinal cord at 3 days after SCI, Scale bar = 10 μm. (G) The tube formation ability of HUVECs, Scale bar = 200 μm. (H) Quantification of tube length from tube formation assay. n≥3, *P<0.05, **P<0.01, ***P<0.001.
Article Snippet: Then, the membranes were blocked with 5% skimmed milk in TBST for 2 h at room temperature, and incubated with the following primary antibodies at 4°C overnight: TRPM2 (1:1000, NB500-242), p-CaMKII (1:1000, sc-32289), CaMKII (1:1000, sc-5306), eNOS (1:1000, sc-376751), NOX2 (1:1000, ab129068),
Techniques: Inhibition, Western Blot, Staining, Tube Formation Assay